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Identification of cholera toxin‐binding sites in the nucleus of intestinal epithelial cells
Author(s) -
Parkinson Marianne E.,
Smith Colin G.,
Garland Peter B.,
van Heyningen Simon
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80491-0
Subject(s) - cholera toxin , toxin , identification (biology) , cholera , nucleus , microbiology and biotechnology , chemistry , microbial toxins , intestinal epithelium , epithelium , biology , biochemistry , genetics , ecology
Post‐embedding immunogold electron microscopy shows several binding sites for cholera toxin in mouse intestinal epithelial cells, particularly in the heterochromatin of the nucleus as well as in the plasma membrane. Anti‐ganglioside GM1 antibodies also bound to the nucleus, but did not interfere with the binding of toxin. 125 I‐labelled toxin bound specifically to a nuclear preparation from rabbit intestinal cells.