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N‐terminal sequencing of photosystem II low‐molecular‐mass proteins 5 and 4.1 kDa components of the O 2 ‐evolving core complex from higher plants
Author(s) -
Ikeuchi Masahiko,
Takio Koji,
Inoue Yorinao
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80482-x
Subject(s) - molecular mass , chloroplast dna , electroblotting , biology , spinach , photosystem ii , dna , chloroplast , homology (biology) , peptide sequence , biochemistry , chemistry , polyacrylamide gel electrophoresis , gene , photosynthesis , enzyme
High resolution gel electrophoresis in the low‐molecular‐mass region combined with electroblotting using polyvinylidene difluoride membranes enabled us to sequence the low‐molecular‐mass proteins of photosystem II membrane fragments from spinach and wheat. The determined N‐terminal sequences, all showing considerable homology between the two plants, involved two newly determined sequences for the 4.1 kDa protein and one for the 5 kDa proteins. The sequence of the 4.1 kDa protein did not match any part of the chloroplast DNA sequence from tobacco or liverwort, suggesting that it is encoded by the nuclear genome. In contrast, the sequence of the 5 kDa protein matched ORF38, which is located just downstream of psb E and psb F in the chloroplast DNA and is assumed to be co‐transcribed with them. These two components were associated with the O 2 ‐evolving core complex. Sequences of other low‐molecular‐mass proteins confirmed the previous identification as photosystem II components.