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Interaction of benz[ a ]pyrene diol epoxide with chromatin studied by flow linear dichroism
Author(s) -
Eriksson S.,
Jernström B.,
Nielsen P.E.,
Nordén B.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80461-2
Subject(s) - chromatin , circular dichroism , pyrene , chemistry , linear dichroism , dna , dichroism , chromophore , linker , stereochemistry , crystallography , biophysics , biochemistry , photochemistry , biology , organic chemistry , physics , computer science , optics , operating system
Chromatin isolated from Ehrlich ascites cells was incubated with the tumourigenic compound (+)‐7β,8α‐dihydroxy‐9α,10α‐epoxy‐7,8,9,10‐tetrahydrobenz[ a ]pyrene [(+)‐ anti ‐BPDE] at low ionic strength and the modified chromatin was analysed using flow linear dichroism (LD). The results confirm that (+)‐ anti ‐BPDE preferentially binds to the DNA in the linker regions, and furthermore show that the long axis of the bound pyrenyl chromophore is oriented parallel or close to parallel to the average orientation of the chromatin fiber axis. The data indicate that the binding geometry of (+)‐ anti ‐BPDE in chromatin is similar to that in pure DNA and deoxyguanosine‐containing double‐helical oligonucleotides.