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Mitochondrial metabolism of guanine nucleotides possible role of guanosine
Author(s) -
Ziegler Mathias,
Dubiel Wolfgang,
Pimenov Alexander M.,
Tikhonov Yuri V.,
Toguzov Rusian T.,
Henke Wolfgang,
Gerber Gerhard
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80457-0
Subject(s) - guanine , guanosine , biochemistry , inosine , hypoxanthine , nucleotide , guanosine diphosphate , chemistry , guanosine triphosphate , mitochondrion , catabolism , organelle , biology , metabolism , adenosine , enzyme , gene
The catabolism of intramitochondrial guanine nucleotides was examined. During 30 min incubation of rat liver mitochondria at 37°C in the presence of oligomycin and carboxyatractyloside, guanine and xanthine were formed and appeared in the medium. Under these conditions, the direct conversion of GMP to guanine by hypoxanthine—guanine phosphoribosyltransferase is suggested to be the main catabolic route within the organelles. Only very small amounts of guanosine were produced and detected both inside and outside the organelles. [ 14 C]Guanosine and [ 14 C]inosine were taken up by the mitochondria. Therefore, guanosine is suggested to be a precursor of intramitochondrial guanine nucleotides.