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The isolation of high molecular mass DNA from the prochlorophyte Prochloron didemni
Author(s) -
Lockhart Peter J.,
Larkum Anthony W.D.,
Beanland Timothy J.,
Howe Christopher J.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80446-6
Subject(s) - genomic dna , molecular cloning , genomic library , restriction enzyme , dna , cloning (programming) , biology , computational biology , endonuclease , microbiology and biotechnology , genetics , chemistry , gene , base sequence , gene expression , computer science , programming language
Using a protocol known to inactivate endogenous nucleases in other organisms we have been able to isolate high molecular mass DNA from Prochloron didemni . This DNA is shown to be suitable for restriction endonuclease analysis and the generation of genomic libraries in the λ cloning vector EMBL3.