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The roles of protein kinase C and cyclic nucleotide dependent kinase in signal transduction in human interferon γ induction by poly I:poly C
Author(s) -
Tamura-Nishimura Motoko,
Sasakawa Shigeru
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80434-x
Subject(s) - protein kinase c , activator (genetics) , signal transduction , cytosol , 12 o tetradecanoylphorbol 13 acetate , protein kinase a , phorbol , stimulation , microbiology and biotechnology , tetradecanoylphorbol acetate , kinase , chemistry , biochemistry , biology , phorbol ester , enzyme , endocrinology , receptor
The signal transduction mechanisms involved in interferon (IFN) γ induction in human peripheral mononuclear lymphocyte nylon‐nonadherent cells (NNA cells) by stimulation with poly(I):poly(C) are investigated. Significant enhancement of IFN γ production by poly(I):poly(C) is observed in the presence of the phorbol ester, 12‐O‐tetradecanoylphorbol 13‐acetate (TPA, a protein kinase C (PKC) activator). Our study shows that in NNA cells, poly(I):poly(C) with or without TPA causes prolonged activation of cytosolic PKC of NNA cells for at least 120 min. The level of activation of PKC is quite remarkable in the case of the combined stimulation by poly(I):poly(C) and TPA as compared to poly(I):poly(C) alone. This demonstrates that prolonged activation of cytosolic PKC for at least 120 min is essential for high levels of production of IFN γ. Moreover, inhibition experiments using the PKC inhibitor H‐7 and cAMP‐dependent protein kinase is inhibitor H‐8 suggest that the mechanism of signal transduction with regard to PKC is involved in stimulation of IFN γ production in NNA cells by poly(I):poly(C) in the presence of TPA and that along with PKC, cAMP‐dependent protein kinase is probably involved in induction of IFN γ by stimulation with poly(I):poly(C) alone.