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Identity of G A1 , G M1a and G D1b synthase in Golgi vesicles from rat liver
Author(s) -
Iber Heinrich,
Kaufmann Reinhard,
Pohlentz Gottfried,
Schwarzmann Günter,
Sandhoff Konrad
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80423-5
Subject(s) - ganglioside , golgi apparatus , vesicle , galactosyltransferase , biochemistry , enzyme , chemistry , golgi membrane , biosynthesis , membrane , biology , cell
Synthesis of ganglioside GD D1b from ganglioside G D2 was demonstrated using Golgi membranes isolated from rat liver. Competition experiments using gangliosides G A2 , G M2 and G D2 as substrates, and as mutual inhibitors for ganglioside galactosyltransferase activity in preparations of Golgi vesicles derived from rat liver, suggested that galactosyl transfer to these three compounds, leading to gangliosides G A1 , G M1a and G D1b respectively, is catalyzed by one enzyme. These results strengthen the hypothesis that the main site for the regulation of ganglioside biosynthesis occurs within the reaction sequence LacCer→G A3 →G D3 →G T3 .