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Mutation in phenol‐type herbicide resistance maps within the psb A gene in Synechocystis 6714
Author(s) -
Ajlani G.,
Meyer I.,
Vernotte C.,
Astier C.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80284-4
Subject(s) - mutant , biology , dcmu , atrazine , point mutation , wild type , asparagine , microbiology and biotechnology , threonine , biochemistry , gene , thylakoid , genetics , amino acid , serine , chloroplast , enzyme , ecology , pesticide
A Synechocystis 6714 mutant resistant to the phenol‐type herbicide ioxynil was isolated and characterized. Sensitivity to DCMU and atrazine was measured in whole cells and isolated thylakoids. The mutant presents the same sensitivity to atrazine as the wild type and a slightly increased sensitivity to DCMU. A point mutation has been found at codon 266 in the psb AI coding locus (AAC to ACC) resulting in an amino acid change from asparagine to threonine in the D 1 protein.