Premium
Leukotriene C 4 inhibits ATP‐dependent transport of glutathione S ‐conjugate across rat heart sarcolemma Implication for leukotriene C 4 translocation mediated by glutathione S ‐conjugate carrier
Author(s) -
Ishikawa Toshihisa
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80278-9
Subject(s) - sarcolemma , glutathione , chemistry , leukotriene c4 , chromosomal translocation , conjugate , leukotriene , biophysics , medicine , biochemistry , biology , enzyme , membrane , mathematical analysis , mathematics , asthma , gene
Sarcolemmal vesicles prepared from rat heart exhibited ATP‐dependent uptake of S ‐(2,4‐dinitrophenyl)glutathione (DNP‐SG), which obeyed Michaelis‐Menten kinetics with an apparent K m of 21 μM for DNP‐SG and a V max of 0.27 nmol · 10 min −1 · mg protein −1 . Several model glutathione S ‐conjugates inhibited DNP‐SG uptake, but leukotriene C 4 inhibited uptake much more significantly even at lower concentrations (competitive inhibition, K i = 1.5 μM). However, leukotrienes D 4 and E 4 , which lack the γ‐glutamyl moiety, were less effective. The results suggest that the ATP‐dependent transport system has a high affinity for leukotriene C 4 , and may be responsible for the translocation of this compound.