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Interaction of BlaI, the repressor for the β‐lactamase gene of Bacillus licheniformis , with the blaP and blaI promoters
Author(s) -
Grossman Matthew J.,
Curran Ivan H.A.,
Lampen J.Oliver
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80258-3
Subject(s) - bacillus licheniformis , repressor , promoter , gene , microbiology and biotechnology , dna footprinting , biology , gene expression , genetics , bacillus subtilis , bacteria
BlaI repressor for the β‐lactamase gene ( blaP ) of Bacillus licheniformis 749, was shown to repress expression of blaP and of the repressor gene ( blaI ), using the purified protein in a DNA‐directed in vitro translation assay. Binding of BlaI to the promoter regions of blaP and blaI was examined by equilibrium and competitive binding assays. BlaI binds to the blaP promoter with an equal or only slightly higher affinity than to the blaI promoter. DNase I footprinting shows that BlaI binds directly to the blaP and blaI promoters, such that RNA polymerase binding and/or transcript elongation would be blocked.