Premium
Human testis cDNA for the regulatory subunit RII α of CAMP‐dependent protein kinase encodes an alternate amino‐terminal region
Author(s) -
Øyen Ole,
Myklebust Frode,
Scott John D.,
Hansson Vidar,
Jahnsen Tore
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80253-4
Subject(s) - protein kinase a , biology , complementary dna , messenger rna , amino acid , microbiology and biotechnology , protein subunit , somatic cell , peptide sequence , kinase , cdna library , gene , biochemistry
Phosphorylations catalysed by cAMP‐dependent protein kinase are essential for sperm motility, and type II cAMP‐dependent protein kinase in mature sperm has been shown to be firmly bound to the flagellum via the regulatory subunit, RII. The present study documents high‐levelled expression of a human, testis‐specific RII α mRNA (2.0 kb) analogous to the rat mRNA which is induced in haploid germ cells [(1988) FEBS Lett. 229, 391–394]. We report the molecular cloning of a full‐length human cDNA corresponding to this unique testis mRNA, and the presence of an alternate amino‐terminal region (amino acids 45–75) of the predicted RII α protein (404 amino acids) compared with the previously published mouse and rat sequences. However, this alternate region is also shown to be present in RII α mRNA (7.0 kb) of human somatic cells. Our data indicate the divergent amino‐terminal sequence to be due to species differences, suggesting an active evolutionary pressure on this particular region, which could be involved in subcellular attachment of RII α and thereby localization of kinase activity to certain targets within the cell.