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Association of phosphatidylinositol kinase and phosphatidylinositol 4‐phosphate kinase activities with the cytoskeleton in human platelets
Author(s) -
Nahas Nabeel,
Plantavid Monique,
Mauco Gérard,
Chap Hugues
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80247-9
Subject(s) - phosphatidylinositol , kinase , inositol , cytoskeleton , biochemistry , microbiology and biotechnology , platelet , chemistry , enzyme , biology , cell , immunology , receptor
The inositol lipid kinases were investigated in the cytoskeletons of human platelets. In the absence of added lipids the kinases were only barely detectable in the Triton‐soluble fractions and undetectable in cytoskeletons of resting cells. However at least 30% of the total phosphatidylinositol kinase was present in the cytoskeleton as revealed by saturation of the enzyme. Phosphatidylinositol 4‐phosphate kinase was also found in significant amounts in the cytoskeletons. On the other hand, both enzymes being only recovered in the particulate fraction of the cells, we suggest that inositol lipid kinases may be present near the anchoring points of the cytoskeletons at the membranes.