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Identification of single‐strand initiation signals in the terC region of the Escherichia coli chromosome
Author(s) -
Hiasa Hiroshi,
Sakai Hiroshi,
Komano Tohru
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80245-5
Subject(s) - biology , escherichia coli , dna , microbiology and biotechnology , genetics , sequence (biology) , duplex (building) , telomere , chromosome , computational biology , gene
On the basis of clear‐plaque formation, we detected initiation signals in the terC region of the Escherichia coli chromosome. At least two single‐strand initiation signals were identified from the terC region. The nucleotide sequences of these two signals were determined. Sequence homologies, variations of the consensus sequence of n′ protein recognition sites, 5′‐GAAGCGG‐3′, were found within these signals. A novel conserved sequence was also found within these signals. Their initiation activities were measured both by the infection growth assay and by the ability to convert the single‐stranded DNA to the duplex replicative form DNA in vivo.