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Studies on the mechanism of inhibition of nuclear triiodothyronine binding by fatty acids
Author(s) -
van der Klis F.R.M.,
Wiersinga W.M.,
de Vijlder J.J.M.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80242-x
Subject(s) - oleic acid , chemistry , triiodothyronine , bovine serum albumin , fatty acid , albumin , biochemistry , mechanism (biology) , binding site , stereochemistry , hormone , philosophy , epistemology
Studies were designed to elucidate the mechanism by which unsaturated fatty acids inhibit the binding of triiodothyronine (T3) ro rat liver nuclei. The possibility of a direct interaction between oleic acid and T3 was excluded by dialysis experiments. Oleic acid inhibits nuclear T3 binding in a strictly competitive manner. The K i value of oleic acid was approx. 10 4 times greater than that of T3. The inhibitory effect of oleic acid could be reversed by bovine serum albumin.