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GTP‐dependent Ca 2+ release from rat liver microsomes Vesicle fusion is not required
Author(s) -
Kleineke Jochen,
Schröder Andrea,
Söling Hans-Dieter
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80236-4
Subject(s) - gtp' , albumin , vesicle , microsome , calcium , chemistry , peg ratio , biochemistry , biophysics , biology , membrane , enzyme , organic chemistry , finance , economics
The GTP‐dependent calcium release from rat liver microsomes is known to be promoted in the presence of colloids like polyethyleneglycol (PEG), polyvinylpyrrolidine, or albumin. Dawson et al. [(1987) Biochem. J. 244, 87–92] using the ‘fusogen’ PEG have concluded that both GTP‐induced calcium efflux and the enhancement of InsP 3 ‐promoted calcium release in the presence of GTP could be attributed to a GTP‐dependent vesicle fusion. Here, using the more physiological colloid albumin we report that GTP‐induced calcium release from rat liver microsomes may not be linked to vesicle fusion.