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The human 32‐kDa stress protein induced by exposure to arsenite and cadmium ions is heme oxygenase
Author(s) -
Taketani Shigeru,
Kohno Hirao,
Yoshinaga Takeo,
Tokunaga Rikio
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80215-7
Subject(s) - sodium arsenite , heme oxygenase , arsenite , heme , chemistry , polyacrylamide gel electrophoresis , biochemistry , gel electrophoresis , hemeprotein , oxygenase , microbiology and biotechnology , cadmium chloride , cadmium , biology , enzyme , arsenic , organic chemistry
Exposure of HeLa and HL60 cells to sodium arsenite or cadmium chloride led to marked increases in cellular heme oxygenase activity. SDS‐polyacrylamide gel clectrophoresis of [ 35S ]methionine‐labeled cellular proteins indicated that these treatments also resulted in the induction of a 32‐kDa protein. Immunoblot analysis further showed that the 32‐kDa protein reacted with anti‐bovine heme oxygenase antibodies. Treatment of the cells with cobaltic chloride or heat induced neither the 32‐kDa protein nor heme oxygenase activity. It is concluded that the 32‐kDa stress protein induced by arsenite and cadmium ions in these human cells is heme oxygenase.