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Reactive oxygen species induce antigenic changes in DNA
Author(s) -
Blount S.,
Griffiths H.R.,
Lunec J.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80200-5
Subject(s) - chemistry , reactive oxygen species , dna , antigen , oxygen , biophysics , microbiology and biotechnology , biochemistry , biology , genetics , organic chemistry
Reactive oxygen species (ROS) are released at sites of inflammation during the respiratory burst which accompanies the phagocytic process. Using an in vitro system to simulate this process we have shown that ROS induce antigenic changes in DNA. More specifically, results of experiments using ROS scavengers have shown that hydroxyl radicals produced in close proximity to DNA‐bound metal ions play a predominant role. ROS‐mediated attack resulted in increased binding of anti‐DNA antibodies to the denatured DNA. These changes were detected using IgG, IgA and IgM isotype binding to antibodies in systemic lupus erythematosus sera. Of these the IgA isotype was most discriminating in its detection of hydroxyl radical‐induced damage.