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Similarities between the regulatory sequences of the unrelated tetracycline genes of pBR322 and TN1O
Author(s) -
Hung A.,
Pictet R.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80191-7
Subject(s) - tn10 , transposable element , pbr322 , genetics , operon , gene , repressor , biology , nucleic acid sequence , promoter , plasmid , escherichia coli , gene expression , genome
The regulatory regions of the tetracycline genes present in pBR322 (pSC101) and in the transposon Tn10 are compared. They show a low degree of nucleotide sequence similarity but a high level of structure similarity. Furthermore, analyses of RNAs transcribed in the opposite direction of the pBR322 tet gene show that there are two mRNA initiation sites separated by 29 nucleotides. This suggests the existence of two promoters for the tet repressor gene in Tn10. These features reveal a strong resemblance of the mode of regulation between the tet operons of Tn10 and pSC101.