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Limited proteolysis of tumor cells increases their plasmin‐binding ability
Author(s) -
Camacho Mercedes,
Fondaneche Marie-Claude,
Burtin Pierre
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80183-8
Subject(s) - plasmin , proteolysis , trypsin , lysine , carboxypeptidase , chemistry , biochemistry , binding site , microbiology and biotechnology , enzyme , biology , amino acid
Mild proteolytic treatment of SW 1116 tumor cells with trypsin or plasmin increases their plasmin‐binding ability considerably by increasing the number of binding sites without altering their affinity. This mechanism may be operative for increasing the concentration of active plasmin at the surface of tumor cells. C‐terminal lysine residues are involved in plasmin binding to cells, since treatment of cells with carboxypeptidase B decreases this binding by 50%.