Premium
Variability in electrophoretic mobility of G i ‐like proteins: effect of SDS
Author(s) -
Jones JoLynda,
Kopf Gregory S.,
Schultz Richard M.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80172-3
Subject(s) - electrophoresis , gel electrophoresis , polyacrylamide gel electrophoresis , gel electrophoresis of proteins , chemistry , two dimensional gel electrophoresis , microbiology and biotechnology , biology , biochemistry , gene , proteomics , enzyme
Two forms of G i ‐like protein are resolved in both somatic cells and mouse gametes when Sigma SDS (95% grade) is used during polyacrylamide gel electrophoresis, whereas only a single species is resolved when Bio‐Rad SDS (electrophoresis grade) is used. These two G i ‐like proteins are likely to reflect two distinct species, since (i) the two species resolved in the presence of Sigma SDS migrate with the same electrophoretic mobility upon re‐electrophoresis in the presence of Sigma SDS and (ii) exchanging Sigma SDS for Bio‐Rad SDS resolves a single species, whereas exchanging Bio‐Rad SDS for Sigma SDS resolves two species.