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1,2‐Diacylglycerol accumulation in human neutrophils does not correlate with respiratory burst activation
Author(s) -
Koenderman Leo,
Tool Anton,
Roos Dirk,
Verhoeven Arthur J.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80170-x
Subject(s) - respiratory burst , diacylglycerol kinase , staurosporine , opsonin , protein kinase c , respiratory system , chemistry , biochemistry , biology , kinase , anatomy , in vitro
Measurements of the level of 1,2‐diacylglycerol (1,2‐DG during activation of the respiratory burst of human neutrophils by formyl‐methionyl‐leucyl‐phenylalanine (fMLP in the presence of platelet‐activating factor (PAF or by opsonized particles show that a correlation between accumulation of 1,2‐DG and O 2 consumption does not exist. Inhibition of protein kinase C activity with staurosporine before addition of opsonized particles demonstrates that the first phase of the respiratory burst is not inhibited, whereas the second phase, which is accompanied by a rise in the content of 1,2‐DG, is strongly inhibited. This study indicates that accumulation of 1,2‐DG cannot be the sole signal for the initiation of the respiratory burst in human neutrophils.