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Supercoil‐stabilized left‐handed DNA in the plasmid (dA‐dT) 16 insert formed in the presence of Ni 2+
Author(s) -
Nejedlý K.,
Kłysik J.,
Paleček E.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80152-8
Subject(s) - insert (composites) , dna supercoil , plasmid , dna , nuclease , chemistry , microbiology and biotechnology , crystallography , z dna , stereochemistry , biophysics , materials science , biology , biochemistry , dna replication , composite material
The (dA‐dT) 16 insert of the plasmid pAT32 was probed with diethyl pyrocarbonate (DEPC) and nuclease Bal 31 in the presence of Ni 2+ known to be able to induce transition to left‐handed conformation in the synthetic poly(dA‐dT)·poly (dA‐T). It has been shown that this insert in a supercoiled plasmid displays a DEPC modification pattern characteristic of left‐handed DNA under conditions not sufficient to induce a left‐handed structure in the linear plasmid and poly(dA‐dT)·poly(dA‐T).