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Identification of the magnesium, europium and lead binding sites in E. coli and lupine tRNA Phe by specific metal ion‐induced cleavages
Author(s) -
Marciniec Tadeusz,
Ciesiołka Jerzy,
Wrzesinski Jan,
Krzyżosiak Włodzimierz J.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80148-6
Subject(s) - transfer rna , hydrolysis , yeast , cleavage (geology) , chemistry , magnesium , biochemistry , escherichia coli , biology , rna , gene , organic chemistry , paleontology , fracture (geology)
The Pb, Eu and Mg‐induced cleavages in E. coli and lupine tRNA Phe have been characterized and compared with those found in yeast tRNA Phe . The pattern of lupine tRNA Phe hydrolysis closely resembles that of yeast tRNA Phe , while several major differences occur in the specificity and efficiency of the E. coli tRNA Phe hydrolysis. The latter tRNA is cleaved with much lower yield in the D‐loop, and interestingly, cleavage is also detected in the variable region, that is highly resistant to hydrolysis in eukaryotic tRNAs. The possible location of tight Pb, Eu and Mg binding sites in E. coli tRNA Phe is discussed on the basis of the specific hydrolysis data.