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Phosphatidylinositol turnover is not a general regulator of neuroblastoma cell differentiation: comparison between two differentiating agents, retinoic acid and γ‐interferon
Author(s) -
Lanciotti M.,
Cornaglia-Ferraris P.,
Ponzoni M.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80146-2
Subject(s) - phosphatidylinositol , retinoic acid , inositol , diacylglycerol kinase , microbiology and biotechnology , cellular differentiation , second messenger system , biology , cell culture , signal transduction , chemistry , biochemistry , receptor , protein kinase c , gene , genetics
The turnover of phosphatidylinositol (PI) is believed to constitute a crucial step in the signaling pathways for stimulation of cells by a variety of bioactive substances, including differentiating agents; however decisive evidence for the idea has not been obtained. In the present paper, we investigated the involvement of PI turnover in cell differentiation using a human neuroblastoma cell line, LAN‐1, which can be induced to differentiate along the neuronal pathway by both retinoic acid (RA) and γ‐interferon (γ‐IFN). Analysis of labelled phosphatidylinositol metabolises from prelabelled cells indicated a rapid decrease of inositol 1,4,5‐trisphosphate and 1,2‐diacylglycerol within 1 min of induction of LAN‐1 cell dilfferentiation by RA, while no changes were observed in γ‐IFN‐treated cells. These findings indicate the occurrence of decreased inositol phospholipid turnover in RA‐treated LAN‐1 cells and suggest that phosphoinositide‐derived metabolises may not constitute general regulators of cellular differentiation.