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Effect of low density lipoproteins, high density lipoproteins, and cholesterol on apolipoprotein A‐I mRNA in Hep G2 cells
Author(s) -
Monge Juan Carlos,
Hoeg Jeffrey M.,
Law Simon W.,
Brewer H.Bryan
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80132-2
Subject(s) - apolipoprotein b , cholesterol , incubation , hep g2 , messenger rna , reverse cholesterol transport , chemistry , endocrinology , medicine , in vitro , intracellular , lipoprotein , biochemistry , biology , gene
We have utilized the human hepatocellular carcinoma cell line, Hep G2, to study the effects of low density lipoproteins (LDL), high density lipoproteins (HDL), and free cholesterol on apolipoprotein (apo) A‐I mRNA levels. Incubation of the Hep G2 cells with LDL and free cholesterol led to a significant increase in the cellular content of cholesterol without any effect on the yield of total RNA or in the cellular protein content. Our studies established that incubation with LDL or free cholesterol increased the relative levels of apoA‐I mRNA in the Hep G2 cells. In contrast with cholesterol loading, HDL had the effect of lowering the levels of apoA‐I mRNA. These results indicate the LDL and HDL pathways as well as intracellular cholesterol may be important in apoA‐I gene expression and regulation.