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Ba 2+ ‐sensitive K + channels in luminal‐membrane vesicles from pars convoluta of rabbit proximal tubule
Author(s) -
Jacobsen Christian,
Røigaard-Petersen Hans,
Sheikh M.I.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80124-3
Subject(s) - vesicle , chemistry , biophysics , membrane , proximal tubule , potassium , choline , membrane potential , biochemistry , biology , endocrinology , kidney , organic chemistry
This paper describes properties of 86 Rb + fluxes through a novel K + channel in luminal‐membrane vesicles isolated from pars convoluta of rabbit proximal tubule. The uptake of 86 Rb + into potassium salt loaded vesicles was specifically inhibited by Ba 2+ . The isotope accumulation is driven by an electrical diffusion potential as shown in experiments using these membrane vesicles loaded with anions of different membrane permeability and was as follows: gluconate > SO 2− 4 > Cl − . Furthermore, the vesicles containing the channels show a cation selectivity with the order K + > Rb + > Li + > Na + = choline + .