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GTP‐dependent and ‐independent activation of superoxide producing NADPH oxidase in a neutrophil cell‐free system
Author(s) -
Ishida Kiyoshi,
Takeshige Koichiro,
Takasugi Shin-ichiro,
Minakami Shigeki
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80123-1
Subject(s) - gtp' , superoxide , cytosol , chemistry , nadph oxidase , biochemistry , cell free system , biophysics , enzyme , biology
GTP and GTP‐γ‐S enhanced several‐fold the NADPH‐dependent superoxide production induced by sodium dodecyl sulfate in a cell‐free system of pig neutrophils consisting of the membrane fraction and two cytosolic fractions separated by gel filtration. The enhanced activity was decreased by the addition of GDP in a dose‐dependent manner, but 70% of the activity in the absence of GTP remained even at 1 mM GDP. Only one cytosol fraction besides the membrane fraction was required for the activation in the presence of GTP. The cytosol fraction was analyzed by chromatography on 2′,5′‐ADP agarose and two components responsible for the GTP‐dependent and independent activation were separated. These results suggest that at least two pathways are available for the activation of superoxide production in the cell‐free system of pig neutrophils.