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Polyamines inhibit the ATP‐dependent proteolytic pathway in rabbit reticulocyte lysates
Author(s) -
Wajnberg Ewa F.,
Fagan Julie M.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80116-4
Subject(s) - reticulocyte , spermine , spermidine , putrescine , biochemistry , chemistry , adenosine triphosphate , proteolysis , enzyme , rna , gene
Reticulocytes contain a soluble nonlysosomal proteolytic pathway that requires ATP and ubiquitin. Polyamines at physiological concentrations were found to inhibit rapidly the ATP‐dependent proteolytic system in reticulocyte lysates; spermidine and putrescine inhibited this process by 26–72% and spermine by 71–96%. Spermine had little effect on the ATP‐independent breakdown of oxidant‐treated hemoglobin. By fractionating the ATP‐dependent system, we show that polyamines inhibit the ATP‐dependent degradation of ubiquitin‐protein conjugates.