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Prediction of terminal protein and ribonuclease H domains in the gene P product of hepadnaviruses
Author(s) -
Khudyakov Yu.E.,
Makhov A.M.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80110-3
Subject(s) - ribonuclease , gene , gene product , ribonuclease iii , microbiology and biotechnology , terminal (telecommunication) , product (mathematics) , genetics , biology , chemistry , virology , rna , gene expression , rna interference , computer science , mathematics , telecommunications , geometry
By means of comparative analysis of primary and secondary structures, and hydropathy plots of hepadnavirus P proteins new functional domains were revealed additionally to the polymerase domain which had been found earlier in these proteins. The C‐terminal part of P proteins was revealed to be significantly similar to ribonuclease H of E. coli . The ribonuclease H functional domain is known to be an integral entity of retrovirus reverse transcriptase as a rule. Availability of this domain indicates once more the putative reverse transcriptase properties of the P products. The proteins of hepadnaviruses were compared to terminal proteins of picornaviruses, adenoviruses and bacteriophages. The data obtained suggested that a conservative N‐terminal region of P proteins functions as protein primer for DNA synthesis in hepadnaviruses.