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Complete sequence of the gene encoding the largest subunit of RNA polymerase I of Trypanosoma brucei
Author(s) -
Jess Waldemar,
Hammer Andrea,
Cornelissen Albert W.C.A.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80029-8
Subject(s) - trypanosoma brucei , gene , biology , rna polymerase , genetics , microbiology and biotechnology , polymerase , rna
We have set out to clone the trypanosomal gene encoding the largest subunit of RNA polymerase I. We screened a genomic library with a synthetic oligonucleotide probe encoding an eleven amino acid sequence motif, YNADFDGDEMN, which has been found in all eukaryotic RNA polymerase largest subunit genes analyzed so far. We isolated the Trp11 locus and determined the complete sequence of the gene encoded within this locus. The deduced amino acid sequence contains the highly conserved RNA polymerase domains as well as the previously identified RNA polymerase I‐specific hydrophilic insertions. Therefore, the gene most closely resembles the largest subunit of RNA polymerase I.