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Purification of squalene‐2,3‐epoxide cyclases from cell suspension cultures of Rabdosia japonica Hara
Author(s) -
Abe Ikuro,
Ebizuka Yutaka,
Seo Shujiro,
Sankawa Ushio
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80024-9
Subject(s) - chemistry , chromatography , cyclase , cycloartenol , epoxide , isoelectric focusing , adenylate kinase , biochemistry , sterol , enzyme , cholesterol , catalysis
Microsomes prepared from cell suspension cultures of Rabdosia japonica Hara showed activities for cyclizing squalene‐2,3‐epoxide into cycloartenol, β‐amyrin and α‐amyrin in the presence of Triton X‐100. These activities were efficiently solubilized by treatment with Triton X‐100 and separated by chromatography on hydroxy apatite, DEAE‐cellulose, isoelectric focusing and gel filtration. The purified cycloartenol cyclase showed a single band on SDS‐polyacrylamide gel electrophoresis with M r = 54000, while β‐amyrin cyclase gave a single band with M r = 28000.