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[ 14 C]Acetate incorporation into glycerolipids from cauliflower proplastids and sycamore amyloplasts
Author(s) -
Alban Claude,
Dorne Albert-Jean,
Joyard Jacques,
Douce Roland
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80023-7
Subject(s) - amyloplast , plastid , chloroplast , biochemistry , biology , diacylglycerol kinase , chromoplast , phosphatidic acid , biosynthesis , botany , phospholipid , enzyme , protein kinase c , membrane , gene
Formation of glycerolipids from [ 14 C]acetate within proplastids from cauliflower buds and amyloplasts from sycamore cells was compared. In cauliflower proplastids, when sn ‐glycerol 3‐phosphate was present, most of the radioactivity was recovered as diacylglycerol, which was converted into MGDG after addition of UDP‐galactose. The MGDG molecular species synthesized in these conditions was C18:1/C16:0 and was rapidly desaturated into C18:3/C16:0. In sycamore amyloplasts, phosphatidic acid, and not diacylglycerol, was the major compound to be synthesized in the presence of sn ‐glycerol 3‐phosphate, therefore, MGDG synthesis from [ 14 C]acetate was not possible in sycamore amyloplasts. We conclude that non‐green plastids almost behave like chloroplasts from C16:3 or C18:3 plants as far as lipid biosynthesis is concerned.