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αB‐Crystallin is expressed in kidney epithelial cell lines and not in fibroblasts
Author(s) -
Nagineni Chandrasekharam N.,
Bhat Suraj P.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80022-5
Subject(s) - fibroblast , cell culture , immunofluorescence , microbiology and biotechnology , biology , antiserum , antibody , immunology , genetics
We have recently shown the presence of αB‐crystallin in non‐ocular tissues of diverse embryological origins such as the heart, brain, spinal cord, kidney, retina, etc. Using an αB‐crystallin‐specific antiserum and immunofluorescence, immunoblotting, immunoprecipitation and peptide mapping with Staphylococcus aureus protease, we demonstrate differential expression of αB‐crystallin in epithelial and fibroblast cell lines. αB‐Crystallin was detectable only in epithelial cell lines such as MDBK, MDCK, LLCPK 1 and JTC‐12, and was not observed in two kidney fibroblast cell lines, one skin fibroblast cell line, and one corneal fibroblast cell line. Differential expression of the αB‐crystallin gene was also confirmed by Northern blot analysis of the RNAs isolated from these cell lines. These data suggest a cell‐type‐specific role for αB.