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Detection of calcium binding by photosystem II polypeptides immobilised onto nitrocellulose membrane
Author(s) -
Webber Andrew N.,
Gray John C.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80020-1
Subject(s) - nitrocellulose , chemistry , photosystem ii , polyacrylamide gel electrophoresis , calcium , biochemistry , gel electrophoresis , membrane , amino acid , peptide sequence , biophysics , biology , photosynthesis , enzyme , gene , organic chemistry
Photosystem II calcium‐binding polypeptides have been detected by their ability to selectively bind 45 Ca when immobilised onto nitrocellulose membrane following SDS‐polyacrylamide gel electrophoresis. Two calcium‐binding polypeptides of 26 kDa and 24 kDa are shown to be components of LHCII. The 24 kDa polypeptide was further characterised by N‐terminal amino acid sequence analysis and shown to be the product of a type II cab gene. A third polypeptide of 33 kDa bound calcium more weakly and was not positively identified.