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The release of nitric oxide from denitrifying cells of Paracoccus denitrificans by an uncoupler is the basis for a new oscillator
Author(s) -
Kučera Igor
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80014-6
Subject(s) - paracoccus denitrificans , cytochrome , nitrite , chemistry , nitric oxide , cytochrome c , denitrifying bacteria , denitrification , biochemistry , photochemistry , nitrate , nitrogen , organic chemistry , enzyme , mitochondrion
Micromolar concentrations of the uncoupler 3‐chlorophenylhydrazonepropanedinitrile caused the release of nitric oxide from the cells of Paracoccus denitrificans respiring nitrite. At the same time, a new component with an absorption maximum at 573 nm could be observed in the difference spectra. Damped oscillations of the concentrations of the cytochrome c ‐NO complex were observed during the trapping of released NO by added mammalian cytochrome c ; reduced cytochrome c accumulated simultaneously in the reaction mixture.