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IS 421 , a new insertion sequence in Escherichia coli
Author(s) -
Sato Showbu,
Nakada Yukari,
Shiratsuchi Akiko
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80007-9
Subject(s) - insertion sequence , escherichia coli , inverted repeat , genetics , direct repeat , biology , nucleic acid sequence , homology (biology) , transposase , orfs , microbiology and biotechnology , transposable element , dna , peptide sequence , amino acid , gene , genome , open reading frame , base sequence
The nucleotide sequence of a new insertion sequence (IS) in Escherichia coli , IS 421 , was determined. It is 1340 bp long and contains inverted repeats of 22 bp at its termini. It is flanked by 13 bp direct repeats apparently generated upon insertion. There are two ORFs longer than 200 bp in IS 421 . One can encode a polypeptide of 371 amino acids (aa) and the other, which is on the other strand, can encode a polypeptide of 102 aa. The C‐terminal part of the 371 aa polypeptide shows some homology to that of transposases encoded in some other known IS elements. The copy number of IS 421 in chromosomal DNA was 4 for E. coli K‐12 and B, and 5 for E. coli C, as determined by the Southern hybridization of restriction fragments.