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Effect of DNA supercoiling on in vitro transcription from the adenovirus early region 4
Author(s) -
Handa Hiroshi,
Watanabe Hajime,
Suzuki Yoshiaki,
Hirose Susumu
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80006-7
Subject(s) - dna supercoil , transcription (linguistics) , dna , microbiology and biotechnology , in vitro , chemistry , biology , genetics , dna replication , linguistics , philosophy
The effect of DNA supercoiling on in vitro transcription from the early region 4 (E4) promoter of adenovirus type 5 (Ad5) has been investigated by using a cell‐free transcription system that maintains supercoiled DNA templates. The supercoiled DNAs yield several‐fold higher levels of E4 transcripts and more faithfully reflect the regulation of in vivo transcription than the linear DNAs.