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ATP synthesis and pyrophosphate‐driven proton transport in tonoplast‐enriched vesicles isolated from Catharanthus roseus
Author(s) -
Dupaix Alain,
Johannin Georges,
Arrio Bernard
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80005-5
Subject(s) - catharanthus roseus , electrochemical gradient , pyrophosphate , inorganic pyrophosphatase , chemiosmosis , ionophore , gramicidin , vesicle , atp synthase , chemistry , adenosine triphosphate , gramicidin s , biochemistry , proton transport , biophysics , membrane , biology , enzyme
In the presence of PP i as an energy source, the tonoplast‐bound inorganic pyrophosphatase from Catharanthus roseus cells is able to create a proton‐gradient which can drive the synthesis of ATP from ADP and P i . ATP synthesis is linked to the pH‐gradient dissipation as monitored by the recovery of the fluorescence intensity of quinacrine and by the amount of synthesized ATP measured by the bioluminescent luciferin/luciferase assay. Proton gradient and ATP synthesis were suppressed by the protonic ionophore gramicidin D.