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Mitochondrial oxidative phosphorylation participating in photosynthetic metabolism of a leaf cell
Author(s) -
Krömer Silke,
Stitt Mark,
Heldt Hans W.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)81453-4
Subject(s) - oligomycin , oxidative phosphorylation , chloroplast , photosynthesis , protoplast , phosphorylation , uncoupling agents , biochemistry , mitochondrion , atp synthase , biology , metabolism , cytosol , microbiology and biotechnology , atpase , enzyme , gene
Oligomycin was used for the selective inhibition of mitochondrial oxidative phosphorylation in barley leaf protoplasts. The addition of oligomycin inhibited the photosynthesis of intact protoplasts by 40–60%, this being accompanied by a pronounced decrease in cellular ATP content. When the protoplasts were ruptured in a way leaving the chloroplasts intact, the photosynthesis of the released chloroplasts was not inhibited by oligomycin. It is therefore concluded that in illuminated protoplasts, ATP formation by oxidative phosphorylation is required for utilization of the photosynthate by the cell. Thus, mitochondrial oxidative phosphorylation appears to serve an essential function for supplying the cytosol with ATP during photosynthesis.