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Sequences both 5′ and 3′ to the transcription initiation site contribute to the ability of a mouse H‐2 gene to respond to type I interferon
Author(s) -
Pascucci Anna,
Pannuti Antonio,
Mantia Girolama La,
Lania Luigi
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)81443-1
Subject(s) - biology , gene , transcription (linguistics) , reporter gene , interferon , genetics , promoter , microbiology and biotechnology , gene expression , regulation of gene expression , regulatory sequence , response element , transfection , philosophy , linguistics
To investigate the cis ‐acting DNA elements that are involved in the regulation of class I major histocompatibility complex genes by interferon, several promoter fragments of the H‐2K k gene were linked to the reporter chloramphenicol acetyl transferase (CAT) gene, and the CAT expression was analyzed in stable transfected cell lines. The functional activities of progressive deletions of the 5′‐flanking region of the H‐2K k gene linked to the CAT gene have allowed us to define a discrete cis ‐acting DNA region necessary for interferon‐mediated stimulation. Moreover, the H‐2K k gene transcribed by the nonregulated SV40 early promoter was also found to be under interferon regulation. Thus interferon enhancement of the H‐2K k gene expression appears to be mediated by two cis ‐acting elements, one located in the 5′‐flanking region and the other by sequences downstream from the transcription initiation site.