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80% of muscarinic receptors expressed by the NB‐OK 1 human neuroblastoma cell line show high affinity for pirenzepine and are comparable to rat hippocampus M1 receptors
Author(s) -
Waelbroeck Magali,
Camus Jean,
Tastenoy Michèle,
Christophe Jean
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)81441-8
Subject(s) - pirenzepine , muscarinic acetylcholine receptor , receptor , muscarinic acetylcholine receptor m1 , muscarinic acetylcholine receptor m2 , endocrinology , medicine , biology , microbiology and biotechnology , muscarinic acetylcholine receptor m3 , muscarinic acetylcholine receptor m4 , chemistry , biochemistry
The NB‐OK 1 human neuroblastoma cell line expressed muscarinic cholinergic receptors that could be labeled with N ‐[ 3 H]methylscopolamine (a nonselective antagonist). 80% of these receptors showed high affinity for pirenzepine, i.e. belonged to the M1 subtype found in neuronal tissues. Their binding properties were identical to those of rat hippocampus M1 receptors, and differed from those of rat pancreas and heart muscarinic receptors. The remaining (20%) muscarinic receptors showed low affinity for pirenzepine and AF‐DX 116, being therefore of an M2β (or B) subtype, and were similar to rat pancreatic receptors.