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Separation of the pore‐forming and cytotoxic activities from natural killer cell cytotoxic factor
Author(s) -
Sashchenko L.P.,
Gnuchev N.V.,
Kirillova M.A.,
Lukjanova T.I.,
Rebizova T.V.,
Revazova E.S.,
Lukanidin E.M.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)81435-2
Subject(s) - cytotoxic t cell , chemistry , natural (archaeology) , biology , biochemistry , in vitro , paleontology
The influence of Ca 2+ on the cytotoxic activity of natural killer cell cytotoxic factor (NKCF) was analyzed. When the natural killer susceptible cell line K562 was exposed to NKCF in the presence of 5 mM Ca 2+ , two peaks of cell damage were found. The first peak was observed after 30–40 min of incubation as a result of pore formation on the surface of target cells. The second was a peak of cytolytic activity which appeared after 24 h of incubation. Upon dilution of the NKCF preparation, only the first peak was observed. Therefore, NKCF produced by large granular lymphocytes in response to K562 consists of different proteins and represents pore‐forming and cytolytic activities.