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Influence of probe structure on unique (regiospecific) cleavage of RNA by RNase H
Author(s) -
Metelev V.G.,
Zayakina G.V.,
Ryabushenko I.L.,
Krynetskaya N.F.,
Romanova E.A.,
Oretskaya T.S.,
Shabarova Z.A.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)81429-7
Subject(s) - rnase p , rna , cleavage (geology) , chemistry , nucleotide , hydrolysis , rnase h , pyrophosphate , stereochemistry , biochemistry , enzyme , biology , gene , paleontology , fracture (geology)
Chimeric oligo(ribo‐deoxyribo)nucleotides with an internucleotide pyrophosphate bond are novel probes for regiospecific hydrolysis of RNA by RNase H. It has been shown that the use of d(TGTGTAT)ppGCCAU leads to unique hydrolysis of the TMV RNA fragment pAAUGGCAUACAC between C 10 and A 11 .