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Photoaffinity labeling of a 33 kDa protein subunit of the δ‐opioid receptor in neuroblastoma and hybrid cell lines
Author(s) -
Icard-Liepkalns C.,
Bochet P.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)81377-2
Subject(s) - neuroblastoma , photoaffinity labeling , receptor , protein subunit , chemistry , affinity label , gel electrophoresis , microbiology and biotechnology , cell culture , opioid receptor , endorphins , biochemistry , opioid , biology , gene , endocrinology , genetics
Tritiated DTLET (Tyr‐D‐Thr‐Gly‐Phe‐Leu‐Thr) binds with high affinity, specificity and saturability to neuroblastoma N18TG 2 and hybrid neuroblastoma × glioma NG108‐15 and NG 108‐5 intact cells. The δ‐opioid receptor density in cells cultured in chemically defined medium was increased about 2 times compared to that in cells cultured in 10% fetal calf serum. A major and a minor protein species covalently and specifically bound to [ 125 I]azido‐DTLET (Tyr‐D‐Thr‐Gly‐pN 3 Phe‐Leu‐Thr), photoactivatable ligand, migrated on SDS‐gel electrophoresis with M r , values near 33000 and 58000, respectively.