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Is translation inhibited by noncognate ternary complexes?
Author(s) -
Bilgin Neşe,
Ehrenberg Måns,
Kurland Charles
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)81362-0
Subject(s) - translation (biology) , ternary complex , ribosome , in vivo , chemistry , ternary operation , stereochemistry , biophysics , biochemistry , biology , genetics , computer science , enzyme , rna , messenger rna , programming language , gene
We studied the influence of an error‐prone isoacceptor (tRNA Leu 4 ), as well as an intermediate (tRNA Leu 2 ) and a weak (tRNA Val ) competitor of tRNA Phe on the poly(Phe) synthesis rate. Even at very high excess concentrations of these noncognate ternary complexes there was no significant effect on the translation rate. Our result argues against the assertion that in vivo translation is slowed down by noncognate tRNA and favours the hypothesis that the incorrect ternary complex concentrations are too low to saturate the ribosomes in vivo.