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Epidermal growth factor‐induced increases in inositol trisphosphates, inositol tetrakisphosphates, and cytosolic Ca 2+ in a human hepatocellular carcinoma‐derived cell line
Author(s) -
Gilligan Ann,
Prentki Marc,
Glen Major C.,
Knowles Barbara B.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)81352-8
Subject(s) - inositol , cytosol , hepatocellular carcinoma , chemistry , cell culture , epidermal growth factor , cancer research , biochemistry , microbiology and biotechnology , biology , receptor , genetics , enzyme
A human hepatocellular carcinoma‐derived cell line, PLC/PRF/5, was examined for its ability to respond to epidermal growth factor (EGF) exposure with increased phosphatidylinositol 4,5‐bisphosphate hydrolysis. Upon addition of EGF (25 ng/ml) a rapid (10–15 s) but transient increase in Ins(1,4,5)P 3 levels and large, prolonged (2 min) increases in Ins(1,3,4,5)P 4 andIns(1,3,4)P 3 levels were detected. Increases in cytosolic Ca 2+ were observed after a 10 to 2O s lag, reaching peak value at 1 min, and remaining elevated for 10 min. The initial burst of cytosolic Ca 2+ occurred in the absence of extracellular Ca 2+ and probably reflects mobilization of intracellular Ca 2+ stores. In cells pretreated with EGTA, the sustained component of the Ca 2+ response was not observed. Comparison of the inositol phosphate and Ca 2+ responses of PLC/PRF/5 cells to responses reported in other cell types indicates that this cell line is a good model for EGF action in liver.