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Immunological studies on the D‐1 and D‐2 proteins of photosystem II preparations from the thermophilic cyanobacterium, Synechococcus sp
Author(s) -
Satoh Kazuhiko,
Dostatni Ralf,
Johanningmeier Udo,
Oettmeier Walter
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)81344-9
Subject(s) - spinach , photosystem ii , synechococcus , photosystem i , coomassie brilliant blue , cyanobacteria , antiserum , dimer , biochemistry , thermophile , chemistry , phycobilisome , photosynthesis , biophysics , biology , enzyme , bacteria , antigen , staining , organic chemistry , genetics
Immunoblotting of Synechococcus photosystem II preparations with site‐directed antisera against the spinach D‐2 protein and an antiserum against a large polypeptide segment of the spinach D‐1 protein showed (i) that the 28 and 31 kDa polypeptides of the cyanobacterial photosystem II core complex are equivalent to the D‐1 and D‐2 proteins of higher plants and algae and (ii) that the β‐octylglucoside treatment of the photosystem II preparation diminishes band intensities of the 28 and 31 kDa proteins in Coomassie blue‐stained gels mainly by increasing the amounts of two proteins which migrate as a dimer of D 1 or D 2 or its aggregate, and of proteolytic digestion products.