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Different sensitivities of the responses of human neutrophils stimulated with immune complex and C5a anaphylatoxin to pertussis toxin
Author(s) -
Shirato Manabu,
Takahashi Kazuhiko,
Nagasawa Shigeharu,
Koyama Jiro
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)81341-3
Subject(s) - anaphylatoxin , immune system , pertussis toxin , extracellular , superoxide , intracellular , immune complex , neutrophil extracellular traps , chemistry , toxin , biology , complement system , immunology , microbiology and biotechnology , inflammation , biochemistry , receptor , enzyme , g protein
When stimulated with immune complex or C5a anaphylatoxin, human neutrophils undergo an increase in the concentration of intracellular Ca 2+ ([Ca 2+ ] i ) that precedes the onset of superoxide (O − 2 ) production. The extracellular Ca 2+ is required for the O − 2 production of neutrophils stimulated by C5a, but is only partially required for those by immune complex. The addition of pertussis toxin to neutrophils does not inhibit the rise in [Ca 2+ ] i and O − 2 production induced by immune complex but does inhibit those induced by C5a. These results suggest that a different sequence of reaction is involved by different stimulants, the anaphylatoxin and immune complex.