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Inaccurate protein synthesis in a mutant of Salmonella typhimurium defective in transfer RNA pseudouridylation
Author(s) -
Negre D.,
Cortay J.C.,
Donini P.,
Cozzone A.J.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)81325-5
Subject(s) - salmonella , mutant , histidine , strain (injury) , transfer rna , rna , protein biosynthesis , biology , gel electrophoresis , microbiology and biotechnology , chemistry , biochemistry , bacteria , gene , genetics , amino acid , anatomy
Protein synthesis was studied comparatively in a wild‐type strain of Salmonella typhimurium and in hisT mutant cells defective in the pseudouridylation of transfer RNA. From a quantitative point of view, no significant difference between the two types of strain was observed when measuring the rate of protein synthesis during either exponential growth or starvation for histidine. In contrast, the qualitative analysis of proteins by two‐dimensional gel electrophoresis showed that histidine‐starved hisT cells mistranslate the genetic program at a higher frequency than exponentially growing hisT cells or either starved or unstarved hisT + cells.