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Isolation and electrophoretic analysis of surface proteins of the zygomycete Absidia glauca
Author(s) -
Teepe Hildegard,
Böttge Jörg-Andreas,
Wöstemeyer Johannes
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)81312-7
Subject(s) - mycelium , hypha , mating type , biology , electrophoresis , isolation (microbiology) , botany , fungus , cell wall , biochemistry , microbiology and biotechnology , gene
Methods for isolation and electrophoretic analysis of surface proteins from the mucoraceous fungus Absidia glauca are described. By analysing the set of cell wall associated proteins which can be extracted with 1 M LiCl, we could show marked differences between submersed and aerial mycelium. Furthermore, during differentiation of the aerial mycelium protein patterns change remarkably. We were able to demonstrate the existence of mating type specific proteins, the most prominent of which is a 15 kDa polypeptide expressed in submersed as well as in aerial hyphae of the (+) mating type.